Rapid and simultaneous genotypic detection of Rifampin-Isoniazid and Ethambutol resistant Mycobacterium tuberculosis by use of MAS-PCR
نویسندگان
چکیده
منابع مشابه
Rapid genotypic detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis directly in clinical specimens.
A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.
متن کاملDetection of Isoniazid and Rifampin Resistant Strain of Mycobacterium Tuberculosis Isolated from patients in Golestan province (North of Iran)
Abstract Background and objectives: With almost nine million new cases each year, tuberculosis is still one of the most Life-threatening diseases in the World. Distribution of drug resistant strains of M.tuberculosis has a lot of importance. This research was carried out to determine the frequency of drug resistance of M. tuberculosis in strains isolated in Golestan province. Material and...
متن کاملrapid detection of rifampicin- and isoniazid-resistant mycobacterium tuberculosis using real-time pcr
conclusions detection of drug resistance associated with mutations through real-time pcr by melting analysis technique showed a high differentiating power. this technique had high concordance with the standard proportion test and mas-pcr results. patients and methods in a cross-sectional study carried out in 2014, 90 patients with m. tuberculosis from five border provinces of iran were selected...
متن کاملSimultaneous ethambutol & isoniazid resistance in clinical isolates of Mycobacterium tuberculosis.
BACKGROUND & OBJECTIVE There is a need to understand the nature of drug resistance patterns and predictors of emergence of drug resistance in Mycobacterium tuberculosis. There could be common factors/mechanisms for resistance to the drugs, isoniazid and ethambutol, both acting on cell wall. The present study was conducted to analyze the antimycobacterial susceptibility patterns of M. tuberculos...
متن کاملDetection of Mycobacterium tuberculosis resistance mutations to rifampin and isoniazid by real-time PCR.
OBJECTIVE The objective of our study was to evaluate the use of a real-time polymerase chain reaction (PCR)-based technique for the prediction of phenotypic resistance of Mycobacterium tuberculosis. MATERIALS AND METHODS We tested 67 M tuberculosis strains (26 drug resistant and 41 drug susceptible) using a method recommended for the LightCycler platform. The susceptibility testing was perfor...
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ژورنال
عنوان ژورنال: International Journal of Mycobacteriology
سال: 2015
ISSN: 2212-5531
DOI: 10.1016/j.ijmyco.2014.10.053